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dc.creatorBobadilla, Fernando Javier
dc.creatorNovosak, Marina Gisel
dc.creatorCortese, Iliana Julieta
dc.creatorDelgado, Osvaldo Daniel
dc.creatorLaczeski, Margarita Ester
dc.date.accessioned2023-08-26T12:25:57Z
dc.date.available2023-08-26T12:25:57Z
dc.date.issued2021-12-30
dc.identifier.citationBobadilla, F.J., Novosak, M.G., Cortese, I.J., Delgado, O.D., Laczeski, M.E. (2022). Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation. BMC Infectious Diseases. Reino Unido : BioMed Central, 21(1), 11 p.es_AR
dc.identifier.issn1471-2334
dc.identifier.other27220
dc.identifier.otherCCPI-CNyE-A-185
dc.identifier.urihttps://hdl.handle.net/20.500.12219/5099
dc.descriptionFil: Bobadilla, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.descriptionFil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.es_AR
dc.descriptionFil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.descriptionFil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.es_AR
dc.descriptionFil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.es_AR
dc.descriptionFil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.es_AR
dc.descriptionFil: Cortese, Iliana Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.es_AR
dc.descriptionFil: Cortese, Iliana Julieta. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.es_AR
dc.descriptionFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.es_AR
dc.descriptionFil: Delgado, Osvaldo Daniel. Universidad Nacional de Catamarca. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.es_AR
dc.descriptionFil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.descriptionFil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.es_AR
dc.descriptionFil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.es_AR
dc.descriptionFil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.es_AR
dc.description.abstractBackground: In pregnant women Streptococcus agalactiae (GBS) can be transmitted to newborn causing severe infections. It is classified into 10 serotypes (Ia, Ib, II-IX). The severity of neonatal disease is determined by the capsular serotype and virulence factors such as the polysaccharide capsule, encoded by the cps gene, protein C, which includes the Cα surface proteins (bca gene), Rib (rib gene) and Cβ (bac gene); the proteins Lmb (lmb gene), FbsB (fbsB gene), FbsA (fbsA gene), the cyl operon encoding a β-hemolysin (hylB gene), the CAMP factor (cfb gene) and the C5a peptidase (scpB gene). The aim of this work was to determine the degree of GBS colonization in pregnant women, the serotypes distribution and to investigate virulence-associated genes. Methods: We worked with 3480 samples of vagino-rectal swabs of women with 35–37 weeks of gestation. The identification of the strains was carried out using conventional biochemical tests and group confirmatory serology using a commercial latex particle agglutination kit. Two hundred GBS strains were selected. Their serotype was determined by agglutination tests. The monoplex PCR technique was used to investigate nine virulence-associated genes (cps, bca, rib, bac, lmb, fbsB, fbsA, hylB and scpB). Results: The maternal colonization was 9.09%. The serotypes found were: Ia (33.50%), III (19.00%), Ib (15.50%), II (14.00%), V (7.00%) and IX (5.50%). 5.50% of strains were found to be non-serotypeable (NT). The nine virulence genes investigated were detected simultaneously in 36.50% of the strains. The genes that were most frequently detected were scpB (100.00%), fbsA (100.00%), fbsB (100.00%), cylB (95.00%), lmb (94.00%) and bca (87.50%). We found associations between serotype and genes bac (p = 0.003), cylB (p = 0.02), rib (p = 0.01) and lmb (p < 0.001). Conclusions: The frequency of vaginal-rectal colonization, serotypes distribution and associated virulence genes, varies widely among geographical areas. Therefore, epidemiological surveillance is necessary to provide data to guide decision-making and planning of prevention and control strategies.es_AR
dc.formatapplication/pdf
dc.format.extent1.3 MB
dc.language.isoenges_AR
dc.publisherBioMed Centrales_AR
dc.relationinfo:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/133884
dc.relationinfo:eu-repo/semantics/altIdentifier/urn/https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-020-05603-5
dc.relationinfo:eu-repo/semantics/altIdentifier/urn/https://link.springer.com/article/10.1186/s12879-020-05603-5
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGBSes_AR
dc.subjectColonizationes_AR
dc.subjectSerotypeses_AR
dc.subjectVirulence geneses_AR
dc.titlePrevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestationes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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