Enterococcus en aguas del arroyo Vicario : recuento, identificación y perfil de sensibilidad
Enterococcus spp in water Vicario stream : Counts, identification and sensibility profiles
Date
2014-06-30Author
Pucciarelli Roman, Amada Beatriz
Tessari, Alberto
Von Specht, Martha Helena
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Enterococcus spp. es uno de los microorganismos indicadores de contaminación fecal de aguas y alimentos. Puede causar infecciones oportunistas en el hombre, y adquirir resistencia a antimicrobianos. El objetivo del estudio fue evaluar la presencia de enterococos en aguas del arroyo Vicario de la ciudad de Posadas, Misiones, y conocer sus características microbiológicas. La detección, caracterización y perfil bioquímico se realizó según normas estándares americanas de aguas. Los ensayos de sensibilidad a quimioterápicos (Glucopeptidos, Aminoglucósidos y ß-lactámicos), según metodología de Clinical Laboratory Standards Institute. Se aislaron 54 cepas: Enterococcus faecalis (86%), E. faecium (9%), E. hirae (1%), E. gallinarum (1%) y otros cocos grampositivos no Enterococcus (3%). Se observó amplia sensibilidad a antibióticos, salvo tres E. faecalis, dos con alto nivel de resistencia a aminoglucósidos y uno con resistencia intermedia a vancomicina. La contaminación provendría de efluentes cloacales considerando el origen intestinal de las cepas aisladas. Los hallazgos de este estudio son relevantes para el control de la resistencia a antibióticos en el medio ambiente. Enterococcus genus is a microorganism considered an indicator of fecal contamination of water and food; their presence in food is due to their ability to survive and their high resistance to different processes and treatment conditions. Enterococcus genus has relevance due to its high incidence in nosocomial illnesses and its property to acquire resistance to antimicrobials agents. The presence of Enterococcus species from Vicario stream water of Posadas City, Misiones, was studied. Detection, characterization and the biochemical profile was done according to the standard methods for water and streams, and sensibility assays to chemotherapeutic (Glucopeptids, Aminoglusides and ß-lactamics were done by the Clinical and Laboratory Standards Institute (CLSI). The strains were: 86% Enterococcus faecalis; 9% E. faecium; 1% E. hirae; 1% E. gallinarum; and 3% no enterococcus. A high sensibility towards antibiotics was observed, except three E. faecalis of which two presented high resistance to aminoglucosides and one with a medium resistance to vancomicine. These contaminants came from waste water considering the intestinal origin of isolated strains. The findings of this study are important for the control of antibiotic resistance in the environment.
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